Preparation and characterization of chitosan polyacrylic. Initial 5fu concentration, the type and concentration of chitosan. A new method for preparation of chitosan microspheres loaded with an hydrophobic drug, ketoprofen, was developed. It is an emulsificationsolvent evaporation process carried out in mild conditions and particularly useful for microencapsulation of thermally sensitive drugs. Microspheres online blog archive chitosan microspheres. Aqueous chitosan solution and ethyl acetate were used as the water and oil phases, respectively. The microspheres were prepared by varying different parameters, e. Preparation of aceclofenac microspheres chitosan solution of varying concentration 1, 2 and 3%wv was prepared in 1%wv acetic acid.
Preparation and evaluation of chitosan microspheres for eradication of helicobacter pylori the main focus of this dissertation is to develop chitosan based microspheres loaded with antibiotic and essential oil to eradicate helicobacter pylori by maintaining the constant drug level and prolonged gastric retention. Design and application of chitosan microspheres as oral and. Carboxymethyl chitosansodium alginate microspheres 4 research efforts in both academic and industrial laboratories. A novel phsensitive hydrogel system composed of a watersoluble chitosan derivative n,ocarboxymethyl chitosan, noccand humanlike collagen hlc blended with phosphate was developed for controlling drug delivery and nocchlc microspheres were prepared by the technology of electrostatic droplet generation. The physicochemical properties of the microspheres related to preparation. Pdf encapsulation of urea was performed in chitosan microspheres via. Porous chitosan microspheres were prepared by freezedrying process based on the interaction between chitosan and tripolyphosphate tpp.
For drugloaded crosslinked chitosan microspheres, the drug and sodium tripolyphosphate, crosslinker were directly dissolved in the chitosan solution before microparticle preparation. Pdf chitosan microspheres of metformin hydrochloride and the. Preparation and characterization of chitosan microsphere loading. Us8153612b2 injectable chitosan mixtures forming hydrogels. A 32 factorial design was employed to study the influence of drug. Preparations, properties and applications of chitosan based. Aceclofenac 5, 10 and 15mg was dispersed in 5ml of this solution and mix well. The drug loaded chitosan microspheres were prepared by dissolving the model drugs 9 or 16. Dissolving chitosan free base in acetic acid produced the chitosan acetate salt.
The optimized microspheres presented average ps of 74461 m and ee. Li deparment of chemistry, sichuan college of education, chendu 610041, sichuan province, china. Chitosan 85% da was procured from marine chemicals, cochin, kerala. Design, optimization and characterizations of chitosan. For that purpose, chitosan microspheres cms in the dispersing state was prepared by the emulsification crosslinking method. Preparation and evaluation of chitosan microspheres for eradication of helicobacter pylori the main focus of this dissertation is to develop chitosan based microspheres. The evaluation of proanthocyanidinschitosanlecithin. Preparation and characterization of chitosankaolinfe 3o4. Chitosan microspheres prepared by spray drying sciencedirect. Preparation and characterization of folatedecorated. Then drug and crosslinking agent were added in each chitosan solution to prepare a uniform dispersion. Hollow chitosan microspheres prepared by an oil1inwater. Pdf composite microspheres based on chitosan and poly.
Preparation of chitosan nanoparticles and nanofibers, including the work achieved. Abstractcrosslinked chitosan microspheres containing 5fluorouracil sfu were prepared. Figure 2 displays scanning electron microscopy sem image of the chitosan microspheres with drug. Preparation of berbamine loaded chitosanagarose microspheres. Chitosanbased floating microspheres of trimetazidin. Design and application of chitosan microspheres as oral. Isoniazid loaded chitosan microspheres for pulmonary. Preparation of magnetic chitosan microspheres and its. The aim of this study is to evaluate, prepare, and characterize bioactivity of chitosan microspheres loaded with bfgf for providing sustained release of bfgf. Chitosan based microspheres of glibenclamide incorporated.
Preparation and characterization of indomethacin loaded. In this investigation, the n, ocarboxymethyl chitosan nocc was synthesized. Chitosancellulose acetate multimicrospheres ccam were prepared by the method of wow emulsion with no toxic reagents and had the size interval of 200280m. Effect of crosslinking agents on chitosan microspheres in.
Microspheres were prepared by the emulsification crosslinking. For the preparation of microspheres, 1%3% wv solutions of chitosan were prepared in acetic acid. Chitosan microsphere used as an effective system to deliver a. The size of final product depends upon the extent of crosslinking agent used while hardening as well as the speed of stirring during the formation of emulsion. Chitosan microspheres loaded with g10e were prepared by.
The microspheres so prepared had a good sphericity and a. Ramaiah college of pharmacy, msrit post, 560054 bangalore, india deveswaran rajamanickam department of pharmaceutics, m. The obtained alginatechitosan microcapsules were used for the preparation of the composite microspheres. Chitosan forms like microspheres, hydrogels, films, nanoparticles, and fibers have been used in biomedical and pharmaceutical applications, include ocular, nasal, oral. All types of microspheres that have been used as nasal drug delivery systems are waterinsoluble but absorb water into the spheres matrix, resulting in swelling of the spheres and the formation of a gel. The resulting microspheres were further solidified by addition of 20 ml of isopropyl alcohol. The above procedure was adopted to obtain microspheres prepared with different concentrations of chitosan and sodium tripolyphosphate table 1. Chitosan based microspheres of glibenclamide incorporated in.
Cross linking chitosan with tpp or encapsulation of bsa does not modify the morphology or size distribution of the chitosan. Preparation of chitosan and watersoluble chitosan microspheres via spraydrying method to lower blood lipids in rats fed with highfat diets yi tao 1, hongliang zhang 1,2, yinming hu 1, shuo wan 1 and zhengquan su 1, 1 key unit of modulating liver to treat hyperlipidpemia satcm and lipid metabolism laboratory. Herein, we present a novel attempt to combine highly immunogenic map from. Chitosan cs is a natural polycationic polymers obtained from. The yield was from 200 mg to 1 g dependent upon the chitosan concentration used. Pdf controlled release of urea from chitosan microspheres. Preliminary trials were carried out to determine the ability of vanillin to crosslink chitosan.
Folic acid chitosan facts and 10hydroxycamptothecin hcptloaded folateconjugated chitosan factshcpt microspheres were prepared by the ionic crosslinking method. Blend hydrogel microspheres of carboxymethyl chitosan. Preparation of alginate coated chitosan microparticles for. The conditions for preparation of the chitosan microparticles with different drug and crosslinker contents are reported in table 1. They were able to achieve a significant change in the zeta potential of their microspheres just by increasing the dosage of chitosan. Preparation and application of chitosan nanoparticles and nanofibers 355 brazilian journal of chemical engineering vol. Chapterv novel blend microspheres of polyvinyl alcohol and. The thermal properties and chemical structure characterization of the cspva blend films were examined by differential scanning calorimetry dsc, thermogravimetric analysis tga, dynamic mechanical analysis dma, and fourier transform infrared ftir. Preparation of berbamine loaded chitosanagarose microspheres and in vitro release study article pdf available in polimeros 225. Processing and characterization of chitosan microspheres to. Chapterv novel blend microspheres of polyvinyl alcohol and succinyl chitosan for controlled release of nifedipine abstract. The drugpolymer dispersion was added in a 150 ml liquid paraffin 100 ml light. Microwave irradiation assisted preparation of chitosan.
Keywords chitosan, drug delivery, microspheres, polyanions, self. The present study aims at the formulation of chitosan microspheres loaded with metformin hydrochloride and to evaluate the effect of different concentration of. Water soluble succinyl chitosan scs was synthesized by reacting succinic anhydride with oh and nh 2 reactive groups of chitosan cs. Hollow chitosan microspheres with and without genipincrosslinking were prepared by an oil 1inwaterinoil 2 emulsion solvent diffusion method without any surfactants and core templates. The chitosan had positive zpotential in the ph range of 58. Preparation of n, ocarboxymethyl chitosanhumanlike. They were stable after sterilization at 121c and 150. Preparation of chitosan microspheres epichlorhydrin crosslinked microspheres ecm a 4% wv chitosan solution was prepared by stirring chitosan in 5% wv acetic acid in a beaker with a magnetic stirrer. Chitosan cellulose acetate microspheres preparation for. In addition, their applications in the wastewater treatment, based on different kinds. Lfloaded cs microspheres were prepared using the spraydrying technique, and then. Dual crosslinked chitosan microspheres formulated with spray.
Thus chemical crosslinking method for preparation of chitosan microspheres involves emulsification followed by crosslinking with a suitable crosslinking agent e. Research article preparation, characterization, and bioactivity of chitosan microspheres containing basic fibroblast growth factor bolv,yuewang,andweichen department of. Characterization chitosan, chitosan nanoparticles, and copperloaded nanoparticles were characterized by following measurements. Microscopic evaluation of microspheres showed that microspheres. Preparation and antibacterial activity of chitosan. Materials 2016, 9, 816 2 of 9 has been extensively applied in agriculture, biotechn ology, biomedicine, and the food industry 3 5. Preparation of alginate microspheres coated with chitosan chitosan was dissolved in 2% acetic acid at a concentration of 0. Ramaiah college of pharmacy, msrit post, 560054 bangalore, india bharath srinivasan. A multiple emulsion method to entrap a lipophilic compound. Preparation of dual crosslinked alginatechitosan blend gel beads. Microspheres were prepared from carboxymethylated chitosan cm. Thus the aim of the present research was to prepare, chitosan microspheres.
Preparation and evaluation of trimetazidine hydrochloride microspheres using chitosan basu s. Morphology, size, encapsulation efficiency and drug release from these microspheres were evaluated. Nov 19, 2008 absorption of antigens onto chitosan microparticles via electrostatic interaction is a common and relatively mild process suitable for mucosal vaccine. The multiple emulsion method produced wellformed microspheres with good yields. Despite the chitosan has been studied in water treatment, their use as microspheres crosslinked with sodium tripolyphosphate in packed columns for removing crude oil from oily waters has not been studied yet. A complete copy of this journal article chitosan microspheres prepared by spray drying is available in. A series of batches of microspheres were prepared by simple emulsification crosslinking method to optimize parameters like external phase mixture of heavy and light liquid paraffin in the ratio of 1. In a randomized, doubleblind intervention trial n 51 in koreans with prediabetes, 12week supplementation with chitosan 1,500 mgday resulted in statistically significantly improved blood glucose at 30 and 60 minutes, glycated hemoglobin hba 1c, change in body fat percentage, and waist circumference compared with the placebo group. Preparation, characterization and crosslinking of chitosan. Preparation and release behavior of carboxymethylated. Vanillin was purchased from merck chemicals corporation ltd. Effect of crosslinking agents on chitosan microspheres in a.
Weigh amount chitosan and 150 mg of drug was dissolved in 15 ml 5% acetic acid. Chitosan forms like microspheres, hydrogels, films, nanoparticles, and fibers have been used in biomedical and pharmaceutical applications, include ocular, nasal, oral, and through the skin. Chitosan microparticles prepared by the waterinoil emulsion. In order to increase the stability of antigens and prevent an immediate desorption of antigens from chitosan carriers in gastrointestinal tract, coating onto bsa loaded chitosan microparticles with sodium alginate was performed by layerby. The highest drug encapsulation efficiencies were obtained for the lowest theoretical drug chitosan. Preparation and evaluation of trimetazidine hydrochloride. Chitosan microspheres applied for removal of oil from. Microspheres are manufactured in both solid and hollow form. Chitosan silica hybrids were synthesized and shaped as microspheres, which may or may not present a coreshell structure, depending on the experimental conditions.
Evaluation of process parameters involved in chitosan. The chitosan solutions to be spray dried were prepared by dissolving chitosan in deionized water containing 0. Preparation and evaluation of microspheres of flurbiprofen html full text. Alginatechitosanplga composite microspheres enabling single. Cross linking chitosan with tpp or encapsulation of bsa does not modify the morphology or size distribution of the chitosan particles, which had an average diameter of 3. Preparation of biocompatible magnetic microspheres with chitosan 195 there is a considerable interest in preparation of magnetite fe3o4 due to its strong magnetic properties, wh. This mixture was added drop wise using a 22gauge hypodermic syringe to the oily phase 100ml of paraffin oil containing 2%wv.
Hollow microspheres are used as additives to lower the density of a material. Interpenetrating polymer network blend microspheres of. Chitosan have properties such as biodegradability, biocompatibility, antimicrobial, nontoxicity, and antitumor due to it utilized in biomedical and pharmaceutical applications. The nicorandilloaded chitosan microspheres were formulated by emulsion crosslinking method. Preparation of chitosanalginateellagic acid sustained. Preparation and evaluation of chitosan microspheres containing. Chitosan and sodium alginate microspheres loaded by aceclofenac were prepared by emulsification and ionic gelation methods. Microspheres are multiparticulate drug delivery systems which are prepared to obtain prolonged or controlled drug delivery to improve bioavailability, stability and to target the drug to specific site at a predetermined rate. Preparation of biocompatible magnetic microspheres with. The cms had smooth surface and spherical shape with the diameter of about 124 m. The building materials in the microspheres have been starch, dextran, albumin. Chitosan microspheres in novel drug delivery systems. Preparation and evaluation of chitosan microspheres. Chitosan microspheres were prepared by a spraydrying technique.
A chitosan composition which forms a hydrogel at near physiological ph and 37 c. Carboxymethyl chitosan cmcs was synthesized and blended with gelatin ge to prepare hydrogel microspheres by wo emulsion. Their structure and morphology were characterized with ir spectroscopy and scanning electron microscopy. Pdf preparation of chitosan particles suitable for enzyme. Chitosan microspheres crosslinked with three different crosslinking agents viz, tripolyphosphate tpp, formaldehyde fa and gluteraldehyde ga have been prepared by spray drying technique. Preparation of chitosan microspheres and modified chitosan microspheres 2. Novel nanoporous magnetic cellulose chitosan composite microspheres nmcms were prepared by solgel transition method using ionic liquids as solvent for the sorption of cuii. Preparation and characterization of chitosanagarose. Preparation and in vitro evaluation of mucoadhesive. Preparation of water in oil emulsion the membrane emulsification, me, process was carried in a commercial unit delivered by micropore ltd.
Bovine serum albumin bsa was encapsulated in the microspheres. Preparation of chitosan particles suitable for enzyme immobilization article pdf available in journal of biochemical and biophysical methods 706. These experiments show the feasibility of preparation of chitosan microparticles in different environments and under different conditions. Preparation and characterization of alginate microspheres. Preparation and evaluation of trospium chloride microspheres using chitosan polymer jhansi reddy k 1,jaya sankar reddy 3,viswanatha reddy v 2,ramesh m y, jeevan kumar 3.
Comparisonof emulsification and ionic gelation method of. Chitosan based floating microspheres of trimetazidin dihydrochloride. The microspheres were incubated with chitosan solution 0. Then drug and crosslinking agent were added in each chitosan. Chitosanreinforced alginate microspheres obtained through the emulsi. Porous chitosansilica hybrid microspheres as a potential. This article was made available online on 16 september 2019 as a fast track article with title. Research article preparation, characterization, and. For this purpose, rivastigmine loaded chitosan microparticles were prepared using the spray drying method. Novel ionic composites based on chitosan cs and clinoptilolite cpl, a widespread natural zeolite, were prepared in this work as microspheres by a tandem ioniccovalent crosslinking. Particularly noteworthy is that chitosan is a promising material for packaging films because of its. Chitosanbased particles as controlled drug delivery systems. Pdf to optimize the preparation process of chitosan microspheres and study its. In this study, we investigated the interface contacting inhibition behaviors of chitosan against bacterial in the dispersing state.
Noncrosslinked and crosslinked chitosan microspheres were prepared by a spray drying method. The entrapment largely depends on the method of preparation and nature of the drug or polymer monomer if used. Preparation, characterization, and bioactivity of chitosan. Chitosanreinforced alginate microspheres obtained through. Preparationandantibacterialactivityofchitosannanoparticles. Preparation and characterization of quaternized chitosan coated alginate microspheres for blue dextran delivery kuoyu chen and siying zeng department of chemical and materials. The samples were prepared by grinding the dry blend microspheres 2 mg with kbr powders 100 mg and then compressing the mixtures to form disks.
Variables believed to be important for microsphere properties were examined. Pdf preparation and characterization of chitosan microsphere. Formulation design for the preparation of indomethacin loaded chitosan microspheres. Chitosan microspheres as delivery drug carriers can be prepared by the waterinoil emulsion solvent diffusion method, being ethyl acetate the oil phase. It was investigated as an ideal alternative to enhancement the adsorption of watersoluble dyes from wastewater. Mucoadhesive chitosan microspheres of amlodipine besylate were prepared for nasal administration with the aim of avoiding the first pass effect.
Preparation and characterization of chitosan polyacrylic acid magnetic microspheres liang guo 1, guang liu 2, ruoyu hong 2,3, and hongzhong li 3. Preparation of crosslinked chitosan microspheres by spray. Spraydried chitosan microspheres crosslinked with d, l. During some research on plga microspheres we found this interesting article published in european cells and materials vol 7 suppl 2. Microspheres are multiparticulate drug delivery systems which are prepared to obtain prolonged or controlled drug delivery to improve bioavailability, stability and to target the drug to specific. The influence of these crosslinking agents on the properties of spray dried chitosan microspheres was extensively investigated. Acetic acid concentration in the polymeric solutions influenced particle size and drug content of the microspheres. The chitosan microspheres were crosslinked to the same level. Various blending ratios of chitosanpoly vinyl alcohol cspva blend films were prepared by solution blend method in this study. The viscosity of the chitosan solutions was measured at 25 1c using a brookfield dviil lvtdvii. All chemicals and reagents used were of analytical grade.
547 335 1035 3 628 560 1585 1413 1313 720 1395 373 780 1638 595 1440 834 936 293 109 1203 89 1325 1590 1591 769 62 982 1031 553 986 207 263 656 973 4 494 1022 452 525 1160 65 781 1087 1190 24 28